JDS4 was a milestone in the series of Joint Danube Surveys.
This document contains the key scientific findings of the Fourth Joint Danube Survey, conducted by many independent teams of different kinds of experts in 13 countries, utilising a new and coordinated data collection approach. It contains 47 chapters, covering a broad range of topics to create an overview of the Danube River Basin.
This report preparation was affected by the pandemic of coronavirus disease in Europe in 2020. The COVID-19 lockdowns adversely impacted the time plan of the analysis of JDS4 samples and consequently some of the results will have to be published later than planned.
Similarly to JDS3, the findings of JDS4 are supportive to the implementation of EU WFD providing an extensive homogeneous dataset acquired by the WFD compliant methods. Even though these data have no ambition to replace the national data used for the assessment of the ecological and chemical status they are an excellent reference database which can be used for WFD assessment methods harmonization throughout the Danube River Basin and for the new derivation and prioritization of the Danube River Basin Specific Pollutants.
JDS4 has provided a great deal of added value to the current monitoring practices in the Danube River Basin, including:
- Independent basin-wide platform for improving national surface water monitoring practices;
- Practical joint testing and comparison of national methodologies for biological and hydromorphological quality elements leading to their future harmonization;
- Interactive platform for hands-on training in sampling and assessment of biological quality elements;
- A unique source of data for a number of quality elements (especially for emerging substances) for the whole Danube;
- Knowledge transfer between EU and non-EU member states.
JDS4 Scientific Report: A Shared Analysis of the Danube River Basin
List of Chapters
- Together for a cleaner and healthier Danube: Joint Danube Survey
- Survey logistics
- Data management
- Recording and assessment of hydromorphological changes 2013 - 2019
- Aquatic macroinvertebrates
- Invasive alien species
- Introduction: (e)DNA-based activities
- Metabarcoding of macrozoobenthos samples
- Metabarcoding of fish eDNA samples
- Metabarcoding of phytobenthos samples
- Metabarcoding of sediment communities
- Summary: performance of (e)DNA-based activities
- Overview chapter on ecology and biology
- Microbial faecal pollution and source tracking
- Occurrence of non-wild type antibiotic resistant Escherichia coli in the River Danube
- Microbiome: Microbial community and environmental DNA analysis
- General physico-chemical determinands and nutrients
- Target analysis of organic substances in water
- Target analysis of organic substances and metals in biota
- Groundwater screening
- Wide-scope target and non-target screening of MAXX large-volume samples of the Danube River with LC-ESI-HRMS and GC-EI-HR-MS
- Wide-scope target and non-target screening of surface water samples by direct injection LC-HRMS techniques
- Screening of endocrine substances in combination with in vitro assays in the Danube River by MAXX large volume solid-phase extraction and LC-HRMS
- Wide-scope target screening of industrial chemicals and plant protection products in wastewater, groundwater, river water, sediments and biota by liquid and gas chromatography coupled with high-resolution mass spectrometry
- Wide-scope target screening of illicit drugs, pharmaceuticals, antibiotics and personal care products in wastewater, groundwater, river water, sediments and biota by liquid chromatography coupled with high resolution mass spectrometry
- Characterization of wastewaters in the Danube River Basin with chemical screening and a battery of in vitro bioassays
- Analysis of organic substances in the Danube River surface water by passive sampling
- Polarity-extended non-target screening using RPLC-HILIC-ESI-QToF-MS/MS and tailor-made data handling
- Comparability of data obtained by suspect and non-target screening and by NORMAN panel of in vitro and in vivo bioassays: results of an interlaboratory study
- Comparison of novel and current approaches for the target- and non-target screening, effect-based monitoring and prioritisation of river basin specific pollutants to improve future water quality monitoring
- Prioritisation of Danube River Basin specific pollutants using the NORMAN prioritisation framework
- Comparison of target screening and target analysis approaches for surface water samples
- Analysis of genotoxic activity of the JDS4 surface water samples collected by horizon large volume solid-phase extraction technique
- Overview chapter on chemical pollution
- Rare earth elements
- Synchronous fluorescence for characterization of dissolved organic matter
- Occurrence of microplastics in the Danube River – A first screening
- Nanoparticle inventory in a sediment core from the Iron Gate I reservoir
- Microplastics in biota – Asian clam case
- Stable isotopes of water and nitrate
JDS4 Scientific Report (Medium Resolution) (21.95 MB) This contains the scientific findings report for JDS4 – one of the most comprehensive investigative surface-water monitoring efforts in the world.
JDS4 Scientific Report (High Resolution) (44.09 MB) This contains the scientific findings report for JDS4 – one of the most comprehensive investigative surface-water monitoring efforts in the world.